quantifluor dsdna sample kit e2671 (Promega)
90
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Promega
quantifluor dsdna sample kit e2671
Quantifluor Dsdna Sample Kit E2671, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quantifluor+dsdna+sample+kit+e2671/quantifluor+dsdna+system/pm37515767-194-126-156
Average 90 stars, based on 1 article reviews
Quantifluor Dsdna Sample Kit E2671, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quantifluor+dsdna+sample+kit+e2671/quantifluor+dsdna+system/pm37515767-194-126-156
Average 90 stars, based on 1 article reviews
quantifluor dsdna sample kit e2671 - by Bioz Stars,
2026-09
90/100 stars
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Recombinant:Article Title: Multiplexed fitness profiling by RB-TnSeq elucidates pathways for lignin-related aromatic catabolism in Sphingobium sp. SYK-6. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial strains Bacterial strains, see Table S9 This study N/A Chemicals and recombinant proteins Kanamycin monosulfate GoldBio K-120-5; CAS: 25389-94-0 2,6-diaminopimelic acid ACROS Organics AC235540010; CAS: 583-93-7 DpnI enzyme New England Biolabs R0176 Q5 HiFi 2X Master Mix New England Biolabs M0492 Ferulic acid Sigma-Aldrich 128708; CAS: 537-98-4 Vanillin Sigma-Aldrich V1104; CAS: 121-33-5 Vanillic acid Sigma-Aldrich 94770; CAS: 121-34-6 4-hydroxybenzoic acid Sigma-Aldrich 240141; CAS: 99-96-7 Syringaldehyde Sigma-Aldrich S7602; CAS: 134-96-3 Methanol Sigma-Aldrich 34860; CAS: 67-56-1 Acetic acid (sodium salt) Sigma-Aldrich S2889; CAS: 127-09-3 Protocatechuic acid ACROS Organics AC114890250; CAS: 99-50-3 Syringic acid AK Scientific C591; CAS: 530-57-4 DDVA Oakwood Chemical 375175; CAS: 2134-90-9 GGE TCI America G0233; CAS: 7382-59-4 Trehalose Research Products International T82000; CAS: 6138-23-4 L-Methionine Sigma-Aldrich M9625; CAS: 63-68-3 b-1 dimer compounds This study CHEBI:86530 Critical commercial assays GeneJet Genomic DNA Purification Kit Thermo Scientific K0721 Monarch Genomic DNA Purification Kit New England Biolabs T3010S QuantiFluor dsDNA Sample Kit Promega E2671 NucleoSpin PCR Clean-Up Kit Macherey-Nagel 740609 Zymoclean Gel DNA Recovery Kit Zymo Research D4007 DNA1000 Kit Agilent 5067–1505 NEBNext Ultra II FS DNA Library Prep Kit with NEBNext Sample Purification Beads New England Biolabs E6177S Deposited data Raw sequencing reads This study SRA: PRJNA905394 Oligonucleotides Oligonucleotides, see Table S9 This study N/A Recombinant DNA Plasmids, see Table S9 This study N/A Software and algorithms RB-TnSeq analysis scripts Wetmore et al.38 https://bitbucket.org/berkeleylab/ feba/src/master/ Custom python scripts for fitness comparisons This study https://github.com/beckham-lab/ SYK-6_RB-TnSeq, https://doi. org/10.5281/zenodo.8066380 16 Cell Reports 42, 112847, August 29, 2023 DNA Purification:Article Title: Multiplexed fitness profiling by RB-TnSeq elucidates pathways for lignin-related aromatic catabolism in Sphingobium sp. SYK-6. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial strains Bacterial strains, see Table S9 This study N/A Chemicals and recombinant proteins Kanamycin monosulfate GoldBio K-120-5; CAS: 25389-94-0 2,6-diaminopimelic acid ACROS Organics AC235540010; CAS: 583-93-7 DpnI enzyme New England Biolabs R0176 Q5 HiFi 2X Master Mix New England Biolabs M0492 Ferulic acid Sigma-Aldrich 128708; CAS: 537-98-4 Vanillin Sigma-Aldrich V1104; CAS: 121-33-5 Vanillic acid Sigma-Aldrich 94770; CAS: 121-34-6 4-hydroxybenzoic acid Sigma-Aldrich 240141; CAS: 99-96-7 Syringaldehyde Sigma-Aldrich S7602; CAS: 134-96-3 Methanol Sigma-Aldrich 34860; CAS: 67-56-1 Acetic acid (sodium salt) Sigma-Aldrich S2889; CAS: 127-09-3 Protocatechuic acid ACROS Organics AC114890250; CAS: 99-50-3 Syringic acid AK Scientific C591; CAS: 530-57-4 DDVA Oakwood Chemical 375175; CAS: 2134-90-9 GGE TCI America G0233; CAS: 7382-59-4 Trehalose Research Products International T82000; CAS: 6138-23-4 L-Methionine Sigma-Aldrich M9625; CAS: 63-68-3 b-1 dimer compounds This study CHEBI:86530 Critical commercial assays GeneJet Genomic DNA Purification Kit Thermo Scientific K0721 Monarch Genomic DNA Purification Kit New England Biolabs T3010S QuantiFluor dsDNA Sample Kit Promega E2671 NucleoSpin PCR Clean-Up Kit Macherey-Nagel 740609 Zymoclean Gel DNA Recovery Kit Zymo Research D4007 DNA1000 Kit Agilent 5067–1505 NEBNext Ultra II FS DNA Library Prep Kit with NEBNext Sample Purification Beads New England Biolabs E6177S Deposited data Raw sequencing reads This study SRA: PRJNA905394 Oligonucleotides Oligonucleotides, see Table S9 This study N/A Recombinant DNA Plasmids, see Table S9 This study N/A Software and algorithms RB-TnSeq analysis scripts Wetmore et al.38 https://bitbucket.org/berkeleylab/ feba/src/master/ Custom python scripts for fitness comparisons This study https://github.com/beckham-lab/ SYK-6_RB-TnSeq, https://doi. org/10.5281/zenodo.8066380 16 Cell Reports 42, 112847, August 29, 2023 Polymerase Chain Reaction:Article Title: Multiplexed fitness profiling by RB-TnSeq elucidates pathways for lignin-related aromatic catabolism in Sphingobium sp. SYK-6. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial strains Bacterial strains, see Table S9 This study N/A Chemicals and recombinant proteins Kanamycin monosulfate GoldBio K-120-5; CAS: 25389-94-0 2,6-diaminopimelic acid ACROS Organics AC235540010; CAS: 583-93-7 DpnI enzyme New England Biolabs R0176 Q5 HiFi 2X Master Mix New England Biolabs M0492 Ferulic acid Sigma-Aldrich 128708; CAS: 537-98-4 Vanillin Sigma-Aldrich V1104; CAS: 121-33-5 Vanillic acid Sigma-Aldrich 94770; CAS: 121-34-6 4-hydroxybenzoic acid Sigma-Aldrich 240141; CAS: 99-96-7 Syringaldehyde Sigma-Aldrich S7602; CAS: 134-96-3 Methanol Sigma-Aldrich 34860; CAS: 67-56-1 Acetic acid (sodium salt) Sigma-Aldrich S2889; CAS: 127-09-3 Protocatechuic acid ACROS Organics AC114890250; CAS: 99-50-3 Syringic acid AK Scientific C591; CAS: 530-57-4 DDVA Oakwood Chemical 375175; CAS: 2134-90-9 GGE TCI America G0233; CAS: 7382-59-4 Trehalose Research Products International T82000; CAS: 6138-23-4 L-Methionine Sigma-Aldrich M9625; CAS: 63-68-3 b-1 dimer compounds This study CHEBI:86530 Critical commercial assays GeneJet Genomic DNA Purification Kit Thermo Scientific K0721 Monarch Genomic DNA Purification Kit New England Biolabs T3010S QuantiFluor dsDNA Sample Kit Promega E2671 NucleoSpin PCR Clean-Up Kit Macherey-Nagel 740609 Zymoclean Gel DNA Recovery Kit Zymo Research D4007 DNA1000 Kit Agilent 5067–1505 NEBNext Ultra II FS DNA Library Prep Kit with NEBNext Sample Purification Beads New England Biolabs E6177S Deposited data Raw sequencing reads This study SRA: PRJNA905394 Oligonucleotides Oligonucleotides, see Table S9 This study N/A Recombinant DNA Plasmids, see Table S9 This study N/A Software and algorithms RB-TnSeq analysis scripts Wetmore et al.38 https://bitbucket.org/berkeleylab/ feba/src/master/ Custom python scripts for fitness comparisons This study https://github.com/beckham-lab/ SYK-6_RB-TnSeq, https://doi. org/10.5281/zenodo.8066380 16 Cell Reports 42, 112847, August 29, 2023 Sample Purification:Article Title: Multiplexed fitness profiling by RB-TnSeq elucidates pathways for lignin-related aromatic catabolism in Sphingobium sp. SYK-6. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial strains Bacterial strains, see Table S9 This study N/A Chemicals and recombinant proteins Kanamycin monosulfate GoldBio K-120-5; CAS: 25389-94-0 2,6-diaminopimelic acid ACROS Organics AC235540010; CAS: 583-93-7 DpnI enzyme New England Biolabs R0176 Q5 HiFi 2X Master Mix New England Biolabs M0492 Ferulic acid Sigma-Aldrich 128708; CAS: 537-98-4 Vanillin Sigma-Aldrich V1104; CAS: 121-33-5 Vanillic acid Sigma-Aldrich 94770; CAS: 121-34-6 4-hydroxybenzoic acid Sigma-Aldrich 240141; CAS: 99-96-7 Syringaldehyde Sigma-Aldrich S7602; CAS: 134-96-3 Methanol Sigma-Aldrich 34860; CAS: 67-56-1 Acetic acid (sodium salt) Sigma-Aldrich S2889; CAS: 127-09-3 Protocatechuic acid ACROS Organics AC114890250; CAS: 99-50-3 Syringic acid AK Scientific C591; CAS: 530-57-4 DDVA Oakwood Chemical 375175; CAS: 2134-90-9 GGE TCI America G0233; CAS: 7382-59-4 Trehalose Research Products International T82000; CAS: 6138-23-4 L-Methionine Sigma-Aldrich M9625; CAS: 63-68-3 b-1 dimer compounds This study CHEBI:86530 Critical commercial assays GeneJet Genomic DNA Purification Kit Thermo Scientific K0721 Monarch Genomic DNA Purification Kit New England Biolabs T3010S QuantiFluor dsDNA Sample Kit Promega E2671 NucleoSpin PCR Clean-Up Kit Macherey-Nagel 740609 Zymoclean Gel DNA Recovery Kit Zymo Research D4007 DNA1000 Kit Agilent 5067–1505 NEBNext Ultra II FS DNA Library Prep Kit with NEBNext Sample Purification Beads New England Biolabs E6177S Deposited data Raw sequencing reads This study SRA: PRJNA905394 Oligonucleotides Oligonucleotides, see Table S9 This study N/A Recombinant DNA Plasmids, see Table S9 This study N/A Software and algorithms RB-TnSeq analysis scripts Wetmore et al.38 https://bitbucket.org/berkeleylab/ feba/src/master/ Custom python scripts for fitness comparisons This study https://github.com/beckham-lab/ SYK-6_RB-TnSeq, https://doi. org/10.5281/zenodo.8066380 16 Cell Reports 42, 112847, August 29, 2023 Sequencing:Article Title: Multiplexed fitness profiling by RB-TnSeq elucidates pathways for lignin-related aromatic catabolism in Sphingobium sp. SYK-6. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial strains Bacterial strains, see Table S9 This study N/A Chemicals and recombinant proteins Kanamycin monosulfate GoldBio K-120-5; CAS: 25389-94-0 2,6-diaminopimelic acid ACROS Organics AC235540010; CAS: 583-93-7 DpnI enzyme New England Biolabs R0176 Q5 HiFi 2X Master Mix New England Biolabs M0492 Ferulic acid Sigma-Aldrich 128708; CAS: 537-98-4 Vanillin Sigma-Aldrich V1104; CAS: 121-33-5 Vanillic acid Sigma-Aldrich 94770; CAS: 121-34-6 4-hydroxybenzoic acid Sigma-Aldrich 240141; CAS: 99-96-7 Syringaldehyde Sigma-Aldrich S7602; CAS: 134-96-3 Methanol Sigma-Aldrich 34860; CAS: 67-56-1 Acetic acid (sodium salt) Sigma-Aldrich S2889; CAS: 127-09-3 Protocatechuic acid ACROS Organics AC114890250; CAS: 99-50-3 Syringic acid AK Scientific C591; CAS: 530-57-4 DDVA Oakwood Chemical 375175; CAS: 2134-90-9 GGE TCI America G0233; CAS: 7382-59-4 Trehalose Research Products International T82000; CAS: 6138-23-4 L-Methionine Sigma-Aldrich M9625; CAS: 63-68-3 b-1 dimer compounds This study CHEBI:86530 Critical commercial assays GeneJet Genomic DNA Purification Kit Thermo Scientific K0721 Monarch Genomic DNA Purification Kit New England Biolabs T3010S QuantiFluor dsDNA Sample Kit Promega E2671 NucleoSpin PCR Clean-Up Kit Macherey-Nagel 740609 Zymoclean Gel DNA Recovery Kit Zymo Research D4007 DNA1000 Kit Agilent 5067–1505 NEBNext Ultra II FS DNA Library Prep Kit with NEBNext Sample Purification Beads New England Biolabs E6177S Deposited data Raw sequencing reads This study SRA: PRJNA905394 Oligonucleotides Oligonucleotides, see Table S9 This study N/A Recombinant DNA Plasmids, see Table S9 This study N/A Software and algorithms RB-TnSeq analysis scripts Wetmore et al.38 https://bitbucket.org/berkeleylab/ feba/src/master/ Custom python scripts for fitness comparisons This study https://github.com/beckham-lab/ SYK-6_RB-TnSeq, https://doi. org/10.5281/zenodo.8066380 16 Cell Reports 42, 112847, August 29, 2023 Software:Article Title: Multiplexed fitness profiling by RB-TnSeq elucidates pathways for lignin-related aromatic catabolism in Sphingobium sp. SYK-6. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial strains Bacterial strains, see Table S9 This study N/A Chemicals and recombinant proteins Kanamycin monosulfate GoldBio K-120-5; CAS: 25389-94-0 2,6-diaminopimelic acid ACROS Organics AC235540010; CAS: 583-93-7 DpnI enzyme New England Biolabs R0176 Q5 HiFi 2X Master Mix New England Biolabs M0492 Ferulic acid Sigma-Aldrich 128708; CAS: 537-98-4 Vanillin Sigma-Aldrich V1104; CAS: 121-33-5 Vanillic acid Sigma-Aldrich 94770; CAS: 121-34-6 4-hydroxybenzoic acid Sigma-Aldrich 240141; CAS: 99-96-7 Syringaldehyde Sigma-Aldrich S7602; CAS: 134-96-3 Methanol Sigma-Aldrich 34860; CAS: 67-56-1 Acetic acid (sodium salt) Sigma-Aldrich S2889; CAS: 127-09-3 Protocatechuic acid ACROS Organics AC114890250; CAS: 99-50-3 Syringic acid AK Scientific C591; CAS: 530-57-4 DDVA Oakwood Chemical 375175; CAS: 2134-90-9 GGE TCI America G0233; CAS: 7382-59-4 Trehalose Research Products International T82000; CAS: 6138-23-4 L-Methionine Sigma-Aldrich M9625; CAS: 63-68-3 b-1 dimer compounds This study CHEBI:86530 Critical commercial assays GeneJet Genomic DNA Purification Kit Thermo Scientific K0721 Monarch Genomic DNA Purification Kit New England Biolabs T3010S QuantiFluor dsDNA Sample Kit Promega E2671 NucleoSpin PCR Clean-Up Kit Macherey-Nagel 740609 Zymoclean Gel DNA Recovery Kit Zymo Research D4007 DNA1000 Kit Agilent 5067–1505 NEBNext Ultra II FS DNA Library Prep Kit with NEBNext Sample Purification Beads New England Biolabs E6177S Deposited data Raw sequencing reads This study SRA: PRJNA905394 Oligonucleotides Oligonucleotides, see Table S9 This study N/A Recombinant DNA Plasmids, see Table S9 This study N/A Software and algorithms RB-TnSeq analysis scripts Wetmore et al.38 https://bitbucket.org/berkeleylab/ feba/src/master/ Custom python scripts for fitness comparisons This study https://github.com/beckham-lab/ SYK-6_RB-TnSeq, https://doi. org/10.5281/zenodo.8066380 16 Cell Reports 42, 112847, August 29, 2023 |